Actividad antimicrobiana in vitro del extracto de hojas de “toronjil” (melissa officinalis) frente a escherichia coli y klebsiella pneumoniae
Fecha
2025-09-08
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Universidad Nacional Intercultural Fabiola Salazar Leguía de Bagua
Resumen
Dado el incremento de la resistencia antimicrobiana y la necesidad de buscar nuevas alternativas terapéuticas, la presente tesis buscó aportar al conocimiento científico en los ámbitos de la medicina herbal y la microbiología. El propósito de la investigación fue determinar el efecto antimicrobiano del extracto de hojas de Melissa officinalis contra cepas de Escherichia coli y Klebsiella pneumoniae, mediante el método difusión en pocillos en agar a concentraciones de 100, 200, 300 y 400 mg/mL, además se comparó con el disco estándar de gentamicina (10 µg). Los resultados de la investigación demostraron el efecto inhibitorio del “toronjil” contra cepas de Escherichia coli y Klebsiella pneumoniae con halos de inhibición de 14.2 y 12.6 mm respectivamente, y una concentración mínima inhibitoria (CMI) que oscilaron entre 6.25 a 25 mg/mL. Se concluyó que el extracto de Melissa officinalis presentó efecto antimicrobiano frente a cepas bacterias estudiadas, dicha evidencia avala el uso de esta planta en el campo de la fitoterapia y la lucha contra las infecciones bacterianas, además podría llevar al desarrollo de nuevos agentes antimicrobianos a partir de compuestos naturales.
Given the increase in antimicrobial resistance and the need to find for new therapeutic alternatives, the present thesis sought to contribute to scientific knowledge in the fields of herbal medicine and microbiology. The purpose of the research was to determine the antimicrobial effect of Melissa officinalis leaf extract against Escherichia coli and Klebsiella pneumoniae strains, using the agar well diffusion method at concentrations of 100, 200, 300 and 400 mg/mL, and compared with the standard gentamicin disk (10 µg). The results of the research demonstrated the inhibitory effect of Melissa officinalis against Escherichia coli and Klebsiella pneumoniae strains with inhibition halos of 14.2 and 12.6 mm respectively, and a minimum inhibitory concentration (MIC) that ranged from 6.25 to 25 mg/mL. It was concluded that the extract of Melissa officinalis did present an antimicrobial effect against the bacterial strains studied. This evidence supports the use of this plant in the field of phytotherapy and the fight against bacterial infections. It could also lead to the development of new antimicrobial agents from natural compounds.
Given the increase in antimicrobial resistance and the need to find for new therapeutic alternatives, the present thesis sought to contribute to scientific knowledge in the fields of herbal medicine and microbiology. The purpose of the research was to determine the antimicrobial effect of Melissa officinalis leaf extract against Escherichia coli and Klebsiella pneumoniae strains, using the agar well diffusion method at concentrations of 100, 200, 300 and 400 mg/mL, and compared with the standard gentamicin disk (10 µg). The results of the research demonstrated the inhibitory effect of Melissa officinalis against Escherichia coli and Klebsiella pneumoniae strains with inhibition halos of 14.2 and 12.6 mm respectively, and a minimum inhibitory concentration (MIC) that ranged from 6.25 to 25 mg/mL. It was concluded that the extract of Melissa officinalis did present an antimicrobial effect against the bacterial strains studied. This evidence supports the use of this plant in the field of phytotherapy and the fight against bacterial infections. It could also lead to the development of new antimicrobial agents from natural compounds.
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Actividad antimicrobiana, Escherichia coli, Extracto de hojas de toronjil, Halos de inhibición, Klebsiella pneumoniae, Antimicrobial activity, Lemon balm leaf extract, Inhibition halos
Citación
Castañeda Terrones, M. S. (2025). Actividad antimicrobiana in vitro del extracto de hojas de “toronjil” (melissa officinalis) frente a escherichia coli y klebsiella pneumoniae. Tesis para optar el título profesional de Biotecnólogo. Escuela Profesional de Biotecnología, Facultad de Ciencias Naturales y Aplicadas, Universidad Nacional Intercultural Fabiola Salazar Leguía de Bagua, Bagua, Perú
